Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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Knowledge-enriched characterization of cell populations. Morphological facts from photographs can insert towards the richness of flow cytometry knowledge. For example, the determine displays an or else standard apoptosis assay working with Annexin V and PI, introducing mobile imaging to characterize cells in Each individual population to reveal morphologically unique attributes. These insights could not are received from multiplex staining by itself.
As samples are acquired within the Attune CytPix Flow Cytometer, the high-speed brightfield camera captures and outlets photographs of detected gatherings in a amount up to 6,000 photos for each 2nd, based on the flow fee and impression size. For increased versatility, Attune Cytometric Computer software enables you to modify the image seize frequency as wanted and select a particular gate for graphic seize, if expected.
Incorporate acoustic concentrating technological know-how with high-parameter Examination for adaptable spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing with a high-pace brightfield digicam for simultaneous substantial throughput flow cytometry and high resolution brightfield imaging.
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In addition, it is illuminating to see the amount of doublets slip by way of standard singlet gates, and excellent to confirm the status of unpredicted double-good gatherings in my Evaluation."
Pair your Attune flow cytometer having an autosampler for enhanced workflow effectiveness, and insert robotic integration for optimum throughput.
Steady graphic high-quality even at superior throughput Acoustic focusing as well as a large-velocity digital camera combine to impression these T cells continually at very low or substantial flow fees. Quickly change concentrate and camera options to fulfill experimental specifications.
The Attune NxT cytometer is definitely the trusted workhorse instrument your lab can depend upon for steady and reliable overall performance. The Attune CytPix product incorporates a superior-pace brightfield digicam and AI-pushed graphic analysis software, with new upgrades including machine Studying tools and an intuitive consumer interface, enabling enhanced info precision, precision, and speedier insights into sample biology.
Most likely most importantly, these gatherings contain cells of Obviously unique phenotypes which could lead on to incorrect conclusions with regards to double favourable read more gatherings (specifically in rare populations).
AI-driven automated image Investigation With all the Attune Cytometric Software package interprets occasion functions into unique morphology parameters which might be combined with common fluorescence and scatter parameters to reveal remarkably reproducible inhabitants adjustments and special subsets.
We Formerly shown the power of imaging Auto-T/Ramos mobile interactions. Let’s examine just the populace of best interest, the double positive functions, to learn more. We can easily now use extended morphological parameters (in this article, circularity and skewness of depth) to more analyze the attributes of such populations and refine gating on these gatherings, expanding data robustness.
Leverage the strength of Highly developed engineering and innovation to accelerate your discoveries. Trust from the Attune Flow Cytometer relatives to deliver the dependability, speed, and precision that established your get the job done aside.
No wash/no lyse leukocyte Investigation with imaging. Cells were being acquired from 24-hour-aged blood by dilution in 1 mM EDTA ( Skilled UX/UI allows consumers to prepare AI products for expanded programs. (A) The “Train Product” feature presents annotation equipment and serious-time design education accuracy.
At the center of every Attune Flow Cytometer lies our groundbreaking acoustic focusing technologies, which makes use of sound waves to meticulously align cells throughout the flow mobile. This modern solution surpasses common hydrodynamic approaches, resulting in faster processing speeds, Remarkable facts excellent, and greater sample throughput.
As the cell line had previously been analyzed to the Attune CytPix Flow Cytometer, the researchers went back again to the pictures and have been able to doc the microbial infection a minimum of five days earlier.
The Attune CytPix Flow Cytometer lets end users to visualise samples during acquisition, validate the precision of gating methods, and support benefits with trustworthy documentation for publication reasons.
What seemed to be a homogeneous populace is really much more diverse—an insight that should be thought of when interpreting effects.